A novel quantitative analysis method for cellulose fibre was developed to understand its behaviour in biological wastewater treatment and waste sludge processes. The method developed in this study was designed using Pseudomonas aeruginosa to remove it by dissolving all the organic components except cellulose from the sludge due to needing the solubilisation of bacteria occupied almost of sludge matrix and quantifying the amount of remaining cellulose. The results of this study indicated that a combined treatment process that employed 2,000 U/L protease, 2 M hydrogen peroxide, and 2 mM potassium hydroxide after pre-treatment for floc dispersion with an ultrasonic treatment at 26 W for 1 min resulted in a solubilisation of 96% of P. aeruginosa without losing the cellulose fibre. When it was applied to the cellulose fibre added in the sludge from a municipal wastewater treatment facility, 99.5% of the cellulose fibre was recovered by using the high-speed centrifuge.
Keywords: Cellulose; fibre; quantitative analysis; toilet paper; waste sludge.