Rapid production of lignocellulolytic enzymes by Trichoderma harzianum LZ117 isolated from Tibet for biomass degradation

Abstract

In this study, Trichoderma harzianum LZ117 was obtained during screening of cellulolytic enzyme producers from samples collected in Tibet. T. harzianum LZ117 exhibits dramatically earlier enzyme induction (48 h) and shorter fermentation time (5 days) during cellulase production when compared with the widely studied strain T. reesei QM9414. Strain LZ117 showed 61% of the maximum cellulase activity at 48 h, whereas only 17% of the maximum cellulase activity was detected in QM9414 at the same culture time. Early induction and rapid production of cellulase were also observed in LZ117 when compared with two other T. harzianum strains. Significantly higher glucose yield was obtained using crude enzyme from strain LZ117 from pretreated corn stover and Jerusalem artichoke stalks when compared with that of T. harzianum reference strain K223452. These results indicate that strain LZ117 is a promising cellulase producer for bioconversion of lignocellulosic biomass.

Keywords: Cellulase production; Induction of cellulase; Lignocellulosic biomass degradation; Submerged fermentation; Trichoderma harzianum.