Biodegradable free-standing films from lauroyl derivatives of hyaluronan

Abstract

Hyaluronan (HA) films exhibit properties suitable for medical applications, but the solubility of HA limits their use in aqueous environments. This can be overcome by modifying HA with hydrophobic side groups that enable physical cross-linking. In this work, we present water insoluble free-standing films from lauroyl modified HA as novel biomaterials with properties tuneable by the degree of HA substitution. The films are homogeneous, mechanically strong, and flexible and can be sterilized by ethylenoxide. To characterize the films, we measured their thickness, dry mass, content of residual organic solvent, mechanical properties, swelling and enzymatic degradation. The safety and biodegradability of the films were tested both in-vitro and in-vivo, showing that the films are safe and that their degradation can be tailored by the degree of HA substitution.

Keywords: Enzymatic degradation; Film; In-vivo evaluation; Lauroyl hyaluronan; Solution casting; Swelling.