Stem Cell Composition of Umbilical Cord Blood Following Milking Compared with Delayed Clamping of the Cord Appears Better Suited for Promoting Hematopoiesis

Anup C Katheria; Ramina Amino; Jason M Konop; Anthony J Orona; Elizabeth Kim; Yang Liu; Donlaong Wu; Evan Y Snyder

doi:10.1016/j.jpeds.2019.07.043

Stem Cell Composition of Umbilical Cord Blood Following Milking Compared with Delayed Clamping of the Cord Appears Better Suited for Promoting Hematopoiesis

J Pediatr. 2020 Jan:216:222-226. doi: 10.1016/j.jpeds.2019.07.043. Epub 2019 Aug 27.

Authors

Anup C Katheria¹, Ramina Amino², Jason M Konop³, Anthony J Orona³, Elizabeth Kim⁴, Yang Liu³, Donlaong Wu³, Evan Y Snyder⁵

Affiliations

¹ Neonatal Research Institute, Sharp Mary Birch Hospital for Women and Newborns, San Diego, CA. Electronic address: anup.katheria@sharp.com.
² Department of Pediatrics, University of California San Diego, La Jolla, CA; Center for Stem Cells and Regenerative Medicine, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA; Sanford Consortium for Regenerative Medicine, La Jolla, CA.
³ Center for Stem Cells and Regenerative Medicine, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA; Sanford Consortium for Regenerative Medicine, La Jolla, CA.
⁴ Center for Stem Cells and Regenerative Medicine, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA.
⁵ Department of Pediatrics, University of California San Diego, La Jolla, CA; Center for Stem Cells and Regenerative Medicine, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA; Sanford Consortium for Regenerative Medicine, La Jolla, CA. Electronic address: esnyder@sbp.edu.

PMID: 31471115
DOI: 10.1016/j.jpeds.2019.07.043

Abstract

In comparing placental transfusion strategies, blood obtained from an umbilical cord that has been "milked" vs one in which clamping was simply delayed contains mesenchymal stromal cells in addition to solely hematopoietic stem cells, a composition more favorable for hematopoiesis, as suggested by its superior rescue of lethally irradiated bone marrow-depleted mice.

Keywords: anemia; delayed cord clamping; hematopoiesis; hematopoietic stem cells; mesenchymal stromal cells; premature infants; umbilical cord blood; umbilical cord milking.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blood Specimen Collection / methods*
Constriction
Fetal Blood / cytology*
Hematopoiesis / physiology*
Hematopoietic Stem Cells / physiology*
Mesenchymal Stem Cells / physiology*
Mice
Time Factors