Alternaria tenuissima infects stored potatoes, and causes tuber rot, resulting in significant economic losses. As a naturally-occurring polysaccharide (poly-β-(1 → 4) N-acetyl-D-glucosamine), chitosan has been reported to be an eco-friendly alternative to synthetic fungicides for the control of postharvest diseases on agricultural commodities. In this study, application of 0.25-1.25 g/L chitosan significantly inhibited spore germination and mycelial growth of A. tenuissima in vitro, with the greatest inhibitory effect observed at the highest concentration. Cytological and biochemical analysis of A. tenuissima spores indicated that exposure to 1.25 g/L chitosan significantly damaged the plasma membrane and increased the level of lipid oxidation. Gene expression analysis in potato tuber revealed that an application of 1.25 g/L chitosan induced the expression of defense-related genes, including catalase, peroxidase, polyphenol oxidase, chitinase and β-1,3-glucanase, and the level of flavonoids and lignin. Chitosan effectively controlled tuber rot caused by A. tenuissima. Collectively, results of the current study indicate that the ability of chitosan to reduce Alternaria rot in stored potato tubers is due to its direct antifungal activity and its ability to induce defense responses in potato tuber tissues. Chitosan may have the potential as a substitute for synthetic fungicides to reduce postharvest losses in potato.
Keywords: Alternaria tenuissima; Antifungal activity; Chitosan; Induced resistance; Potato tuber.
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