In this study, we reported a new micropillar array electroporation (MAE) platform to accomplish large-scale, size-specific DNA and RNA delivery to mammalian cells for nanomedicine. By introducing well-patterned micropillar array on the electrode surface, the number of micropillars each cell faces varies with the surface area of cell membrane or the size of cells. In this way, cell size-specific electroporation is conveniently done on a large population of cells in despite of their random locations between the two electrodes. The enhancement of this MAE system on the delivery of DNA and RNA probes without sacrifice of cell viability is demonstrated with an average increase of 2.5 to 3-fold on the transfection efficiency of DNA plasmids and additional knockdown of the targeted protein 10-55% more in siRNA delivery when compared to that using a commercial electroporation system. This MAE system works like many single cell electroporation are carried out in parallel, showing potential to bridge the gap between single cell electrophysiology study and in vitro electroporation to a large population of cells.
Keywords: Electroporation; Gene delivery; Micropillar array; Microstructured electrode; Transfection enhancement.