Scallop Extracts Inhibited LPS-Induced Inflammation by Suppressing MAPK and NF-κB Activation in RAW264.7 Macrophages

Adv Exp Med Biol. 2019:1155:1069-1081. doi: 10.1007/978-981-13-8023-5_88.

Abstract

Scallops belong to cosmopolitan family of bivalves which are found in any oceans. They are one of the most important marine fishery resources in the world. The shell, meat and pearl layer have a high utilization value and a lot of scallops are eaten as food. In this study, we established anti-inflammatory effect of Scallops water extract in lipopolysaccharide (LPS) stimulated RAW 264.7 mononuclear macrophage. Our results indicated that Scallop water extract effectively reduced the synthesis of nitric oxide (NO). In addition, Scallop water extract suppressed the reactive oxygen species (ROS) generation and the expression of IL-6 and TNF-α. Further investigation indicated that anti-inflammatory effect of Scallop water extract via suppressing downregulation of MAPK (JNK, p38 and ERK) and NF-κB signaling.

Keywords: Inflammation; MAPK and NF-κB signaling; Macrophages; Scallop extracts.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Cell Extracts / pharmacology*
  • Cytokines / metabolism
  • Inflammation
  • Lipopolysaccharides
  • Macrophages / drug effects*
  • Mice
  • Mitogen-Activated Protein Kinases / metabolism*
  • NF-kappa B / metabolism*
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase Type II / metabolism
  • Pectinidae / chemistry*
  • RAW 264.7 Cells
  • Reactive Oxygen Species / metabolism

Substances

  • Anti-Inflammatory Agents
  • Cell Extracts
  • Cytokines
  • Lipopolysaccharides
  • NF-kappa B
  • Reactive Oxygen Species
  • Nitric Oxide
  • Nitric Oxide Synthase Type II
  • Nos2 protein, mouse
  • Mitogen-Activated Protein Kinases