Protective effects of raspberry on the oxidative damage in HepG2 cells through Keap1/Nrf2-dependent signaling pathway

Lei Chen; Kang Li; Qian Liu; José L Quiles; Rosanna Filosa; Mohammad Amjad Kamal; Fang Wang; Guoyin Kai; Xiaobo Zou; Hui Teng; Jianbo Xiao

doi:10.1016/j.fct.2019.110781

Protective effects of raspberry on the oxidative damage in HepG2 cells through Keap1/Nrf2-dependent signaling pathway

Food Chem Toxicol. 2019 Nov:133:110781. doi: 10.1016/j.fct.2019.110781. Epub 2019 Aug 26.

Authors

Lei Chen¹, Kang Li², Qian Liu³, José L Quiles⁴, Rosanna Filosa⁵, Mohammad Amjad Kamal⁶, Fang Wang⁷, Guoyin Kai⁸, Xiaobo Zou⁹, Hui Teng¹⁰, Jianbo Xiao¹¹

Affiliations

¹ College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: chenlei841114@hotmail.com.
² College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: likang007@gmail.com.
³ College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: qian_liu1@163.com.
⁴ Department of Physiology, Institute of Nutrition and Food Technology "Jose Mataix", Biomedical Research Centre, University of Granada, Armilla, 18100, Granada, Spain. Electronic address: jlquiles@ugr.es.
⁵ Department of Experimental Medicine, University of Campania, Via L. De Crecchio 7, Naples, 80138, Italy. Electronic address: rosanna.filosa@unicampania.it.
⁶ King Fahd Medical Research Center, King Abdulaziz University, P. O. Box 80216, Jeddah, 21589, Saudi Arabia; Enzymoics, 7 Peterlee Place, Hebersham, NSW, 2770, Australia; Novel Global Community Educational Foundation, Australia. Electronic address: prof.ma.kamal@gmail.com.
⁷ College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, 210023, China. Electronic address: wangfang8875@163.com.
⁸ Laboratory of Medicinal Plant Biotechnology, College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 311402, China. Electronic address: kaiguoyin@163.com.
⁹ International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang, 212013, China. Electronic address: zou_xiaobo@ujs.edu.cn.
¹⁰ College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: tenghui850610@126.com.
¹¹ International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang, 212013, China. Electronic address: jianboxiao@yahoo.com.

PMID: 31465820
DOI: 10.1016/j.fct.2019.110781

Abstract

The aim of the present study was to explore the protective effects of raspberry and its bioactive compound cyanidin 3-O-glucoside against H₂O₂-induced oxidative stress in HepG2 cells. We established a model of oxidative stress in HepG2 cells induced by H₂O₂ and examined the protein expression of Keap1/Nrf2. The antioxidant activity of raspberry extract was carried out measuring the level of reactive oxygen species (ROS), and the changes of phase II detoxification elements such as GSH level and CAT activity. Also the expression of proteins related to the Keap1/Nrf2 signaling was tested. The results revealed that raspberry extract significantly reduced the ROS levels in oxidative injured cells, increased GSH content and CAT activity, and activated the expression of proteins Keap1, Nrf2, HO-1, NQO1, and γ-GCS. These results taken together indicated that raspberry treatment could ameliorate H₂O₂-induced oxidative stress in HepG2 cells via Keap1/Nrf2 pathway.

Keywords: Cyanidin 3-O-Glucoside; HepG2 cells; Keap1/Nrf2 pathway; Oxidative damage; Raspberry.

MeSH terms

Anthocyanins / pharmacology
Antioxidants / pharmacology*
Fruit / chemistry
Glucosides / pharmacology
Hep G2 Cells
Humans
Hydrogen Peroxide / pharmacology
Kelch-Like ECH-Associated Protein 1 / metabolism*
NF-E2-Related Factor 2 / metabolism*
Oxidative Stress / drug effects*
Plant Extracts / pharmacology*
Rubus / chemistry
Signal Transduction / drug effects*

Substances

Anthocyanins
Antioxidants
Glucosides
KEAP1 protein, human
Kelch-Like ECH-Associated Protein 1
NF-E2-Related Factor 2
NFE2L2 protein, human
Plant Extracts
cyanidin-3-O-beta-glucopyranoside
Hydrogen Peroxide