Abstract
The aim of this work was to investigate the enzyme inhibition, antioxidant activity, and phenolic compounds of Lecokia cretica (Lam.) DC. Acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glycosidase enzymes were strongly inhibited by the L. cretica extracts. IC50 values for the three enzymes were found as 3.21 mg/mL, 2.1 mg/mL, and 2.07 mg/mL, respectively. Antioxidant activities were examined in both aqueous and ethanol (EtOH) extracts using CUPRAC, FRAP, and DPPH method. Also, the phenolic compounds of the endemic plant were identified and quantified by using HPLC/MS/MS. According to the results, the extracts have remarkable antioxidant activities. The most abundant phenolic acids of L. cretica in EtOH extract were determined as quinic acid (12.76 mg/kg of crude extract), chlorogenic acid (3.39 mg/kg), and malic acid (2.38 mg/kg).
Keywords:
HPLC/MS/MS; Lecokia cretica; anticholinergic activity; antidiabetic activity; antioxidant activity.
© 2019 Wiley-VHCA AG, Zurich, Switzerland.
MeSH terms
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Acetylcholinesterase / metabolism
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Antioxidants / chemistry
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Antioxidants / isolation & purification
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Antioxidants / pharmacology*
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Apiaceae / chemistry
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Butyrylcholinesterase / metabolism
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Cholinergic Antagonists / chemistry
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Cholinergic Antagonists / isolation & purification
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Cholinergic Antagonists / pharmacology*
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Cholinesterase Inhibitors / chemistry
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Cholinesterase Inhibitors / isolation & purification
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Cholinesterase Inhibitors / pharmacology
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Dose-Response Relationship, Drug
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Glycoside Hydrolase Inhibitors / chemistry
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Glycoside Hydrolase Inhibitors / isolation & purification
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Glycoside Hydrolase Inhibitors / pharmacology
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Glycoside Hydrolases / metabolism
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Hypoglycemic Agents / chemistry
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Hypoglycemic Agents / isolation & purification
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Hypoglycemic Agents / pharmacology*
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Phenols / chemistry
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Phenols / isolation & purification
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Phenols / pharmacology*
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Phytochemicals / chemistry
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Phytochemicals / isolation & purification
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Phytochemicals / pharmacology*
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Structure-Activity Relationship
Substances
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Antioxidants
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Cholinergic Antagonists
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Cholinesterase Inhibitors
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Glycoside Hydrolase Inhibitors
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Hypoglycemic Agents
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Phenols
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Phytochemicals
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Plant Extracts
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Acetylcholinesterase
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Butyrylcholinesterase
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Glycoside Hydrolases