Effect of temperature on toxicity and biodegradability of dissolved organic nitrogen formed during hydrothermal liquefaction of biomass

Abstract

This study investigated the nutrient content and reuse potential of wastewater generated during hydrothermal liquefaction of microalgal biomass. The hydrothermal liquefaction reaction was tested at 270, 300, 330, and 345 °C to determine the effect of temperature on the formation of non-biodegradable dissolved organic nitrogen (nbDON). Total nitrogen, ammonium, color, and toxicity were selected as key characteristics for the reuse of hydrothermal liquefaction wastewater. Results indicated that a higher concentration of nbDON₅ (nbDON defined with a 5 day growth assay) and more diverse heterocyclic N-containing organic compounds were associated with greater toxicity as measured by a growth rate assay. For the tested temperature ranges, the total nitrogen content of the hydrothermal liquefaction wastewater slightly decreased from 5020 ± 690 mg L^-1 to 4160 ± 120 mg L^-1, but the % nbDON₅ fraction increased from 57 ± 3 %DON to 96 ± 5 %DON. The temperature of hydrothermal liquefaction reactions can be optimized to maximize carbon conversion and nitrogen recovery.

Keywords: HTL wastewater; Heterocyclic N-Containing organic compounds (HNOCs); Hydrothermal liquefaction (HTL); Nitrogen recovery; Non-biodegradable dissolved organic nitrogen (nbDON); Toxicity.