A versatile and accessible polymer coating for functionalizable zwitterionic quantum dots with high DNA grafting efficiency

Chloé Grazon; Margaret Chern; Katherine Ward; Sébastien Lecommandoux; Mark W Grinstaff; Allison M Dennis

doi:10.1039/c9cc04856a

A versatile and accessible polymer coating for functionalizable zwitterionic quantum dots with high DNA grafting efficiency

Chem Commun (Camb). 2019 Sep 21;55(74):11067-11070. doi: 10.1039/c9cc04856a. Epub 2019 Aug 27.

Authors

Chloé Grazon¹, Margaret Chern², Katherine Ward³, Sébastien Lecommandoux⁴, Mark W Grinstaff⁵, Allison M Dennis⁶

Affiliations

¹ Univ. Bordeaux, CNRS, Bordeaux INP, LCPO, UMR 5629, F-33600, Pessac, France and Department of Chemistry, Boston University, Boston, MA, USA.
² Division of Materials Science & Engineering, Boston University, Boston, MA, USA. aldennis@bu.edu.
³ Department of Biomedical Engineering, Boston University, Boston, MA, USA.
⁴ Univ. Bordeaux, CNRS, Bordeaux INP, LCPO, UMR 5629, F-33600, Pessac, France.
⁵ Department of Chemistry, Boston University, Boston, MA, USA and Department of Biomedical Engineering, Boston University, Boston, MA, USA.
⁶ Division of Materials Science & Engineering, Boston University, Boston, MA, USA. aldennis@bu.edu and Department of Biomedical Engineering, Boston University, Boston, MA, USA.

PMID: 31453992
DOI: 10.1039/c9cc04856a

Abstract

Efficient and versatile functionalization of poly(anhydride maleic-alt-isobutylene) (PIMA), with economical commercial reagents, results in the one-step/one-day production of a copper-free click chemistry-ready carboxybetaine-like coating for quantum dots (QDs). The QDs are bright and stable in aqueous media and easily grafted with DNA with >95% efficiency.

MeSH terms

Click Chemistry
Cycloaddition Reaction
Cyclooctanes / chemistry
DNA, Complementary / chemistry
DNA, Complementary / genetics
DNA, Single-Stranded / chemistry*
DNA, Single-Stranded / genetics
Histamine / chemistry
Maleic Anhydrides / chemical synthesis
Maleic Anhydrides / chemistry*
Nucleic Acid Hybridization
Polymers / chemical synthesis
Polymers / chemistry*
Quantum Dots / chemistry*

Substances

Cyclooctanes
DNA, Complementary
DNA, Single-Stranded
Maleic Anhydrides
Polymers
poly(isobutylene-alt-maleic anhydride)
Histamine