Here, we report an integrated operation of microfluidic pumps and valves only by finger actuation. As the working principle of the finger-actuated microfluidic pumps includes deflection of the poly(dimethylsiloxane) (PDMS) membrane, the pneumatic valves for controlling the flow direction can be easily integrated with the pumps. Using a single button, the flow path can be determined and flow generation can be achieved. We also verified the integrated operation of finger-actuated pumps and valves by demonstrating nucleic acid purification.