Abstract
The detailed pathogenesis of endometriosis remains largely unclear despite decades of research. Recent studies have demonstrated that miRNAs plays an important role in endometriosis. The expression of miR-142-3p was decreased in ectopic endometrial tissues, while KLF9 and VEGFA expression levels were increased. Overexpression of miR-142-3p or knockdown of KLF9 significantly suppressed CRL-7566 cell proliferation and metastasis, induced cell apoptosis, and decreased both cell autophagy and vascularization. Additionally, KLF9 was confirmed to be a direct target of miR-142-3p and to directly bind to the promoter of the VEGFA gene, regulating its expression. Finally, intraperitoneal injection of miR-142-3p lentivirus significantly attenuated ectopic endometriotic lesions in vivo.miR-142-3p directly targeted KLF9, regulated VEGFA expression, and was protective against the growth of ectopic endometriotic lesions. Therefore, the miR-142-3p/KLF9/VEGFA signalling pathway may be a potential target in endometriosis treatment.
Keywords:
Endometriosis; KLF9; VEGFA; autophagy; miR-142-3p.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / genetics
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Autophagy / genetics*
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Base Pairing
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Base Sequence
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Cell Line
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Cell Proliferation
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Choristoma / genetics*
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Choristoma / metabolism
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Choristoma / pathology
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Disease Models, Animal
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Endometriosis / genetics*
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Endometriosis / metabolism
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Endometriosis / pathology
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Endometrium / metabolism
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Endometrium / pathology
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Epithelial Cells / cytology
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Epithelial Cells / metabolism
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Female
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Gene Expression Regulation
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Humans
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Kruppel-Like Transcription Factors / genetics*
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Kruppel-Like Transcription Factors / metabolism
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MicroRNAs / genetics*
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MicroRNAs / metabolism
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Neovascularization, Pathologic / genetics*
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Neovascularization, Pathologic / metabolism
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Neovascularization, Pathologic / pathology
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Promoter Regions, Genetic
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Protein Binding
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Rats
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Rats, Sprague-Dawley
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Signal Transduction
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Vascular Endothelial Growth Factor A / genetics*
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Vascular Endothelial Growth Factor A / metabolism
Substances
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KLF9 protein, human
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Kruppel-Like Transcription Factors
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MIRN142 microRNA, human
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MicroRNAs
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VEGFA protein, human
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Vascular Endothelial Growth Factor A
Grants and funding
This work was supported by the Science and Technology Planning Projects of Guangzhou City (China, 2016201604030009).