Purpose: To investigate the effect and molecular mechanism of miR-490 in prostate cancer.
Methods: qRT-PCR was used to detect the expression of miR-490 in human prostate cancer PC-3 cell line (the control group B) and normal human prostate cells (the control group A), the overexpression vector (the positive group) and human prostate cancer PC-3 cells transfected by negative control (the negative group) were built, and the results of the transfection were detected by qRT-PCR.
Results: The relative expression levels of miR-490 in the cells in the control group A, the control group B and the negative group were lower than that in the positive group (p<0.05). The OD values of the cells in the negative and positive groups both increased continuously over time (p<0.05). The OD values of the cells in the positive group at each time point were lower than those in the negative group (p<0.05).
Conclusion: The low expression and overexpression of miR-490 in prostate cancer may inhibit the proliferation, migration and invasion of prostate cancer cells and promote apoptosis by inhibiting the phosphorylation of AKT signal pathway.