Colorimetric and Fluorimetric DNA Detection with a Hydroxystyryl-Quinolizinium Photoacid and Its Application for Cell Imaging

Avijit Kumar Das; Sergey I Druzhinin; Heiko Ihmels; Mareike Müller; Holger Schönherr

doi:10.1002/chem.201903017

Colorimetric and Fluorimetric DNA Detection with a Hydroxystyryl-Quinolizinium Photoacid and Its Application for Cell Imaging

Chemistry. 2019 Oct 1;25(55):12703-12707. doi: 10.1002/chem.201903017. Epub 2019 Sep 9.

Authors

Avijit Kumar Das¹, Sergey I Druzhinin¹, Heiko Ihmels¹, Mareike Müller¹, Holger Schönherr¹

Affiliation

¹ Department of Chemistry and Biology, and Center of Micro- and Nanochemistry and Engineering (Cμ), University of Siegen, Adolf-Reichwein-Str. 2, 57068, Siegen, Germany.

Abstract

The combination of styryl dye properties with the acidity and strong photoacidity of the 2,2'-[(1''-hydroxy-4''-methyl-(E)-2'',6''-phenylene)]-bisquinolizinium enables the detection of DNA by distinct absorption and emission color changes and the fluorimetric detection of DNA in cells with epifluorescence and confocal fluorescence microscopy.

Keywords: bioorganic chemistry; fluorescent probes; heterocycles; live-cell imaging; photoacids.

MeSH terms

Colorimetry / methods*
DNA / chemistry*
Fluorescence
Fluorometry / methods*
Molecular Structure

Substances

DNA

Abstract

MeSH terms

Substances

Grants and funding