Draft genome sequence data of Clostridium thermocellum PAL5 possessing high cellulose-degradation ability

Eiko Nakazono-Nagaoka; Takashi Fujikawa; Ayumi Shikata; Chakrit Tachaapaikoon; Rattiya Waeonukul; Patthra Pason; Khanok Ratanakhanokchai; Akihiko Kosugi

doi:10.1016/j.dib.2019.104274

Draft genome sequence data of Clostridium thermocellum PAL5 possessing high cellulose-degradation ability

Data Brief. 2019 Jul 15:25:104274. doi: 10.1016/j.dib.2019.104274. eCollection 2019 Aug.

Authors

Eiko Nakazono-Nagaoka¹, Takashi Fujikawa², Ayumi Shikata¹, Chakrit Tachaapaikoon³, Rattiya Waeonukul³, Patthra Pason³, Khanok Ratanakhanokchai⁴, Akihiko Kosugi¹

Affiliations

¹ Biological Resources and Post-Harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Japan.
² Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), Japan.
³ Pilot Plant Development and Training Institute (PDTI), King Mongkut's University of Technology Thonburi (KMUTT), Thailand.
⁴ Enzyme Technology Laboratory, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi (KMUTT), Thailand.

Abstract

Clostridium thermocellum is a potent cellulolytic bacterium. C. thermocellum strain PAL5, was derived from strain S14 that was isolated from bagasse paper sludge, possesses higher cellulose-degradation ability than representative strains ATCC27405 and DSM1313. In this work, we determined the draft genome sequence of C. thermocellum PAL5. Genomic DNA was used for whole-genome sequencing using the Illumina HiSeq 2500. We obtained 215 contigs of >200 bp (N50, 78,366 bp; mean length, 17,378 bp). The assembled data were subjected to the National Center for Biotechnology Information (NCBI) Prokaryotic Genome Annotation Pipeline, and 3198 protein-coding sequences, 53 tRNA genes, and 4 rRNA genes were identified. The data are accessible at NCBI (the accession number SBHL00000000). Our data resource will facilitate further studies of efficient cellulose-degradation using C. thermocellum.

Keywords: Cellulose; Cellulose-degradation; Clostridium thermocellum; Draft genome sequence.