Cell stimulation by focused electron beam of atmospheric SEM

Wataru Inami; Daiki Horiba; Yoshimasa Kawata

doi:10.1016/j.ultramic.2019.112823

Cell stimulation by focused electron beam of atmospheric SEM

Ultramicroscopy. 2019 Nov:206:112823. doi: 10.1016/j.ultramic.2019.112823. Epub 2019 Aug 1.

Authors

Wataru Inami¹, Daiki Horiba², Yoshimasa Kawata³

Affiliations

¹ Shizuoka University, Graduate School of Science and Technology, Hamamatsu, 4328561, Japan; Shizuoka University, Research Institute of Electronics, Hamamatsu, 4328011, Japan. Electronic address: inami.wataru@shizuoka.ac.jp.
² Shizuoka University, Graduate School of Science and Technology, Hamamatsu, 4328561, Japan.
³ Shizuoka University, Graduate School of Science and Technology, Hamamatsu, 4328561, Japan; Shizuoka University, Research Institute of Electronics, Hamamatsu, 4328011, Japan.

PMID: 31398577
DOI: 10.1016/j.ultramic.2019.112823

Abstract

Cell stimulation has been performed with a focused electron beam. To protect the live cells from the vacuum environment of the electron beam, the beam irradiated the ambient cells via a thin film. In this way, the cells were electrically stimulated with nanometre resolution in a non-contact process. The response of calcium ion concentration in a single HeLa cell after electron beam irradiation was examined. This technique has the potential to stimulate single ion channels, granules, and organelles.

Keywords: Atmospheric SEM; Cell stimulation; Irradiated damage.

MeSH terms

Calcium / metabolism*
Cell Line, Tumor
Electrons
HeLa Cells
Humans
Microscopy, Electron, Scanning / methods
Organelles / metabolism*
Organelles / physiology*
Vacuum

Substances

Calcium