RNA imaging by chemical probes

Fumiaki Tomoike; Hiroshi Abe

doi:10.1016/j.addr.2019.08.001

RNA imaging by chemical probes

Adv Drug Deliv Rev. 2019 Jul:147:44-58. doi: 10.1016/j.addr.2019.08.001. Epub 2019 Aug 6.

Authors

Fumiaki Tomoike¹, Hiroshi Abe²

Affiliations

¹ Research Center for Materials Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan.
² Department of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan. Electronic address: h-abe@chem.nagoya-u.ac.jp.

PMID: 31398387
DOI: 10.1016/j.addr.2019.08.001

Abstract

Sequence-specific detection of intracellular RNA is one of the most important approaches to understand life phenomena. However, it is difficult to detect RNA in living cells because of its variety and scarcity. In the last three decades, several chemical probes have been developed for RNA detection in living cells. These probes are composed of DNA or artificial nucleic acid and hybridize with the target RNA in a sequence-specific manner. This hybridization triggers a change of fluorescence or a chemical reaction. In this review, we classify the probes according to the associated fluorogenic mechanism, that is, interaction between fluorophore and quencher, environmental change of fluorophore, and template reaction with/without ligation. In addition, we introduce examples of RNA imaging in living cells.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Fluorescent Dyes
Nucleic Acid Hybridization
RNA Probes*
RNA*

Substances

Fluorescent Dyes
RNA Probes
RNA