First identification of Sapoviruses in wild boar

Risako Katsuta; Fujiko Sunaga; Toru Oi; Yen Hai Doan; Satoko Tsuzuku; Yoshihisa Suzuki; Kaori Sano; Yukie Katayama; Tsutomu Omatsu; Mami Oba; Tetsuya Furuya; Yoshinao Ouchi; Junsuke Shirai; Tetsuya Mizutani; Tomoichiro Oka; Makoto Nagai

doi:10.1016/j.virusres.2019.197680

First identification of Sapoviruses in wild boar

Virus Res. 2019 Oct 2:271:197680. doi: 10.1016/j.virusres.2019.197680. Epub 2019 Aug 6.

Authors

Risako Katsuta¹, Fujiko Sunaga¹, Toru Oi², Yen Hai Doan³, Satoko Tsuzuku⁴, Yoshihisa Suzuki⁵, Kaori Sano⁶, Yukie Katayama⁷, Tsutomu Omatsu⁷, Mami Oba⁷, Tetsuya Furuya⁸, Yoshinao Ouchi⁴, Junsuke Shirai⁸, Tetsuya Mizutani⁷, Tomoichiro Oka⁹, Makoto Nagai¹⁰

Affiliations

¹ School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252-5201, Japan.
² Faculty of Bioresources and Environmental Science, Ishikawa Prefectural University, Nonoichi, Ishikawa 921-8836, Japan.
³ Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan.
⁴ Kenpoku Livestock Hygiene Service Center, Mito, Ibaraki 310-0002, Japan.
⁵ Mie Chuo Livestock Hygiene Service Center, Tsu, Mie 514-0061, Japan.
⁶ Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan; Department of Pathology, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-0052, Japan.
⁷ Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
⁸ Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
⁹ Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan. Electronic address: oka-t@nih.go.jp.
¹⁰ School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252-5201, Japan; Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan. Electronic address: m-nagai@azabu-u.ac.jp.

PMID: 31398366
DOI: 10.1016/j.virusres.2019.197680

Abstract

Sapoviruses (SaVs) are enteric viruses that have been detected in human and animals previously; however, SaVs have not been identified in wild boar yet. Using a metagenomics approach, we identified SaVs in fecal samples of free-living wild boars in Japan for the first time. Six of the 48 specimens identified belonged to one genogroup (G)III, one GV and four GVI SaV sequence reads. We successfully determined complete genome of GV and GVI SaV strains using the long reverse transcription PCR strategy and the 5' rapid amplification of cDNA end method. Phylogenetic tree analysis and pairwise distance calculation revealed that GV SaV detected from wild boar was related to recently assigned GV.5 strains from pig, while GVI SaV was assigned to a new genotype within GVI. Moreover, wild boar may act as a reservoir for transmission of SaVs to the pig population (and vice versa) because GIII, GV, and GVI SaVs were all detected in pigs previously.

Keywords: complete genome; new genotype; sapovirus; wild boar.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Genome, Viral*
Genomics / methods
Genotype*
Japan
Phylogeny
Sapovirus / classification*
Swine
Vesicular Exanthema of Swine / virology*