Abstract
In recent years cancer immunotherapy, especially the cell-based immunotherapy, has reached several milestones and achieved a lot of cancer remission in the clinics. Obtaining a more potent and effective cytotoxic T lymphocytes (CTLs) for cancer immunotherapy is always the ultimate goal for the researchers. However, the difficulty in harvesting a large number of tumor antigen-specific CTLs from the tumor patient is still a major obstacle we need to overcome. In our previous studies, it is shown that pluripotent stem cell-derived CTL-especially the genetically engineered antigen-specific CTLs-may serve as a good source of unlimited number of highly reactive and antigen-specific CTLs. Here we present a two-step method for the generation of antigen-specific T lymphocytes from iPS cells by in vitro priming and in vivo maturation.
Keywords:
Differentiation; Induced pluripotent stem cells; Notch signaling; Stem cell biology; T cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Neoplasm / genetics
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Antigens, Neoplasm / immunology
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Calcium-Binding Proteins / genetics
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Calcium-Binding Proteins / metabolism
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Cell Culture Techniques / instrumentation
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Cell Culture Techniques / methods*
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Cell Differentiation
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Cell Line, Tumor
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Coculture Techniques / instrumentation
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Coculture Techniques / methods
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Culture Media / metabolism
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Disease Models, Animal
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Female
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Flow Cytometry / instrumentation
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Flow Cytometry / methods
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Genetic Vectors / genetics
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Homeodomain Proteins / genetics
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Humans
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Immunotherapy, Adoptive / methods*
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Induced Pluripotent Stem Cells / physiology*
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Melanoma, Experimental / immunology
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Melanoma, Experimental / therapy*
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Mice
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Mice, Knockout
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Protein Engineering / methods
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Receptors, Antigen, T-Cell / genetics
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Receptors, Antigen, T-Cell / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Retroviridae / genetics
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T-Lymphocytes, Cytotoxic / physiology
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T-Lymphocytes, Cytotoxic / transplantation*
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Transduction, Genetic / instrumentation
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Transduction, Genetic / methods
Substances
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Antigens, Neoplasm
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Calcium-Binding Proteins
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Culture Media
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Dlk1 protein, mouse
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Homeodomain Proteins
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Receptors, Antigen, T-Cell
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Recombinant Proteins
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RAG-1 protein