Fluorescence excitation-emission matrices were measured for 111 samples of different types of beer and studied by the parallel factor analysis (PARAFAC). The 5-component PARAFAC model was found to suitably describes the beer fluorescence, accounting for 99.4% of the fluorescence variance in the measured set of samples, and providing the completely resolved excitation and emission spectra of each component. The model was chosen based on a model's core consistency and split-half analysis. It is shown that beer fluorescence is the sum of fluorescence of aromatic amino acids (tryptophan, tyrosine, and phenylalanine), different forms of vitamin B, and phenolic compounds. Obtained PARAFAC model of beer fluorescence demonstrated the potential for the quantification and quality analysis of beer fluorophores and classification of different beer types.
Keywords: Beer; Excitation-emission matrices; Fluorescence; PARAFAC; PLS-DA.