Real-time Quantitative PCR Arrays for Virally-associated Cancers

Dirk P Dittmer; Wolfgang Vahrson; Michelle R Staudt; James F Papin; Rebecca Hines-Boykin; Farnaz D Fakhari

Real-time Quantitative PCR Arrays for Virally-associated Cancers

Cancer Genomics Proteomics. 2004 Mar-Apr;1(2):117-124. Epub 2004 Mar 1.

Authors

Dirk P Dittmer¹, Wolfgang Vahrson¹, Michelle R Staudt¹, James F Papin¹, Rebecca Hines-Boykin¹, Farnaz D Fakhari¹

Affiliation

¹ Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, U.S.A.

PMID: 31394676

Abstract

Virally-associated cancers are unique in that their origin is typically well defined and suitable to genomic analysis on a smaller scale. We recently reported the transcription profile of Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) in Kaposi's sarcoma (KS) using a real-time quantitative PCR (QPCR) array. This review explores the advantages and limitations of such an approach as well as the possibilities of extending PCR-based profiling to human cancers. Since real-time QPCR records a truly quantitative transcription profile, this technology will improve statistical analysis and solidify clinical decision-making.

Publication types

Review