Exploring biostimulation of plant hormones and nitrate supplement to effectively enhance biomass growth and lutein production with thermo-tolerant Desmodesmus sp. F51

Nur Rashid Ahmed; Emmanuel Manirafasha; Xueshan Pan; Bor-Yann Chen; Yinghua Lu; Keju Jing

doi:10.1016/j.biortech.2019.121883

Exploring biostimulation of plant hormones and nitrate supplement to effectively enhance biomass growth and lutein production with thermo-tolerant Desmodesmus sp. F51

Bioresour Technol. 2019 Nov:291:121883. doi: 10.1016/j.biortech.2019.121883. Epub 2019 Jul 25.

Authors

Nur Rashid Ahmed¹, Emmanuel Manirafasha², Xueshan Pan¹, Bor-Yann Chen³, Yinghua Lu¹, Keju Jing⁴

Affiliations

¹ Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China.
² Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China; University of Rwanda, College of Education, Kigali, Rwanda.
³ Department of Chemical and Material Engineering, National Ilan University, Yilan 26047, Taiwan.
⁴ Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China. Electronic address: jkj@xmu.edu.cn.

PMID: 31387052
DOI: 10.1016/j.biortech.2019.121883

Abstract

In this study, the interactive effect of plant hormone-salicylic acid and succinic acid on biomass growth, lutein content, and productivity of Desmodesmus sp. F51 were investigated. The results demonstrated that the synergistic action of salicylic acid and succinic acid could effectively enhance the assimilation of nitrate and significantly improve lutein production. The maximal lutein content 7.01 mg/g and productivity 5.11 mg/L/d could be obtained with a supplement of 100 µM salicylic acid and 2.5 mM succinic acid in batch culture. Furthermore, operation strategy of nitrate fed-batch coupled with supplementation for succinic acid and salicylic acid resulted in further enhancement of lutein content and productivity by 7.50 mg/g and 5.78 mg/L/d, respectively. The performance is better than most of the previously reported values.

Keywords: Desmodesmus sp. F51; Lutein; Nitrate fed-batch; Salicylic acid; Succinic acid.

MeSH terms

Biomass*
Lutein / biosynthesis*
Nitrates / pharmacology*
Plant Growth Regulators / pharmacology*

Substances

Nitrates
Plant Growth Regulators
Lutein