Antiviral activity of merimepodib against foot and mouth disease virus in vitro and in vivo

Mol Immunol. 2019 Oct:114:226-232. doi: 10.1016/j.molimm.2019.07.021. Epub 2019 Aug 3.

Abstract

Foot and mouth disease virus (FMDV), a member of family Picornaviridae, belongs to the genus Aphthovirus, which causes foot and mouth disease (FMD), a highly transmissible disease that affects cloven-hoof animals. In spite of the fact that efficient vaccines are available, effective antiviral molecules for FMD are needed to reduce viral infection during early stages of infection. In this study, merimepodib was found to efficiently inhibit FMDV replication in a dose-dependent manner. The 50% inhibitory concentration (IC50) of merimepodib antiviral activity against two distinct FMDV strains (O/MYA98/BY/2010 and A/GD/MM/CHA/2013) was estimated to be 7.859 and 2.876 μM, respectively, while the 50% cytotoxic concentration (CC50) of merimepodib was found to be 47.74 μM. Furthermore, treatment with 30 μg merimepodib efficiently prolonged the survival time of suckling mice infected with FMDV. Taken together, these results suggested that merimepodib has the potential to be a novel antiviral agent against FMDV.

Keywords: Antiviral activity; FMDV; In vivo; Merimepodib.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology*
  • Carbamates / pharmacology*
  • Cell Line
  • Foot-and-Mouth Disease / drug therapy*
  • Foot-and-Mouth Disease Virus / drug effects*
  • Mice
  • Phenylurea Compounds / pharmacology*
  • Swine
  • Viral Vaccines / pharmacology
  • Virus Replication / drug effects

Substances

  • Antiviral Agents
  • Carbamates
  • N-3-(3-(3-methoxy-4-oxazol-5-ylphenyl)ureido)benzylcarbamic acid tetrahydrofuran-3-yl ester
  • Phenylurea Compounds
  • Viral Vaccines