The nanobubbles generated in water are large in non-surface aspect that allows the formation of radicals on their surface. Nanobubbles maintain high pressure inside, and are capable of Brownian motion Most of nanobubbles based research focused on the characteristic of nanobubble to maintain high pressure inside while it shrunk. High temperature was generated when bubble was collapsed by an external impact. In this study, hydrogen nanobubbles were produced based on a self-technique that could store nanobubbles for a long period. The test was conducted to destroy nanobubbles in hydrogen nanobubble water. Ultrasonic waves were applied into the water to destroy nanobubbles and there was rise in water temperature, which was caused by the collapsing effect of bubbles. When ultrasonic waves were applied, the collapsing effect of bubbles was largely exhibited within the initial one minute of the experiment. It was confirmed that the wattage of ultrasonic wave was significantly affected. If the collapsing effect of nanobubbles can be measured, it will be possible to apply this method positively to various fields.