Polyketides from Alternaria alternata MT-47, an endophytic fungus isolated from Huperzia serrata

Fitoterapia. 2019 Sep:137:104282. doi: 10.1016/j.fitote.2019.104282. Epub 2019 Aug 2.

Abstract

Four new polyketides, alternatains A-D (1-4), along with 17 known compounds (5-21) were obtained from the solid substrate fermentation cultures of Alternaria alternata MT-47, an endophytic fungus isolated from the medicinal plant of Huperzia serrata. Their structures were elucidated by extensive spectroscopic and spectrometric techniques (1D and 2D NMR, IR, and HRESIMS) and calculated electronic circular dichroism (ECD) method. Compounds 4, 6, 15, and 21 exhibited inhibitory activities on ATP release of thrombin-activated platelets with IC50 values in the range of 18.2-68.8 μM.

Keywords: Alternaria alternata; Anti-platelet activity; Endophytic fungus; Huperzia serrata; Polyketides.

MeSH terms

  • Acetylcholinesterase
  • Adenosine Triphosphate
  • Alternaria / chemistry*
  • Anticoagulants / isolation & purification
  • Anticoagulants / pharmacology*
  • Blood Platelets / drug effects*
  • Butyrylcholinesterase
  • China
  • Cholinesterase Inhibitors
  • Endophytes / chemistry
  • Humans
  • Huperzia / microbiology*
  • Molecular Structure
  • Plants, Medicinal / microbiology
  • Polyketides / isolation & purification
  • Polyketides / pharmacology*

Substances

  • Anticoagulants
  • Cholinesterase Inhibitors
  • Polyketides
  • Adenosine Triphosphate
  • Acetylcholinesterase
  • Butyrylcholinesterase