Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

J Vis Exp. 2019 Jul 22:(149). doi: 10.3791/59629.

Abstract

Chimeric antigen receptor T (CAR-T) cell therapy is a cutting edge and potentially revolutionary new treatment option for cancer. However, there are significant limitations to its widespread use in the treatment of cancer. These limitations include the development of unique toxicities such as cytokine release syndrome (CRS) and neurotoxicity (NT) and limited expansion, effector functions, and anti-tumor activity in solid tumors. One strategy to enhance CAR-T efficacy and/or control toxicities of CAR-T cells is to edit the genome of the CAR-T cells themselves during CAR-T cell manufacturing. Here, we describe the use of CRISPR/Cas9 gene editing in CAR-T cells via transduction with a lentiviral construct containing a guide RNA to granulocyte macrophage colony-stimulating factor (GM-CSF) and Cas9. As an example, we describe CRISPR/Cas9 mediated knockout of GM-CSF. We have shown that these GM-CSFk/o CAR-T cells effectively produce less GM-CSF while maintaining critical T cell function and result in enhanced anti-tumor activity in vivo compared to wild type CAR-T cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • CRISPR-Cas Systems*
  • Gene Editing*
  • Gene Knockout Techniques
  • Granulocyte-Macrophage Colony-Stimulating Factor / genetics*
  • Granulocyte-Macrophage Colony-Stimulating Factor / physiology
  • Humans
  • Immunotherapy, Adoptive*
  • Neoplasms / therapy*
  • Receptors, Chimeric Antigen / immunology*
  • T-Lymphocytes / immunology*

Substances

  • Receptors, Chimeric Antigen
  • Granulocyte-Macrophage Colony-Stimulating Factor