Abstract
The Streptococcus lactis gene coding for alpha-acetolactate decarboxylase (ADC) was cloned in Escherichia coli. Subsequent subcloning in E. coli showed that the ADC gene was located within a 1.3-kilobase DNA fragment. The ADC gene was controlled by its own promoter. Gas chromatography showed that S. lactis and the transformed E. coli strains produced the two optical isomers of acetoin in different ratios.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetoin / metabolism
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Carboxy-Lyases / genetics*
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Chromosome Mapping
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Cloning, Molecular*
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Genes, Bacterial
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Lactates / metabolism
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Lactococcus lactis / enzymology
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Lactococcus lactis / genetics*
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Stereoisomerism
Substances
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Lactates
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alpha-acetolactate
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Acetoin
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Carboxy-Lyases
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acetolactate decarboxylase