Secretion and functional expression of Mycobacterium bovis antigens MPB70 and MPB83 in lactic acid bacteria

Tuberculosis (Edinb). 2019 Jul:117:24-30. doi: 10.1016/j.tube.2019.05.007. Epub 2019 May 29.

Abstract

The aim of this study was to determine the reliability of lactic acid bacteria (LAB) as heterologous hosts for the expression of MPB70 and MPB83, two Mycobacterium bovis antigens that possess diagnostics and immunogenic properties, respectively. We therefore generated recombinant cells of Lactococcus lactis and Lactobacillus plantarum that carried hybrid genes encoding MPB70 and MPB83 fused to signal peptides that are specifically recognized by LAB. Only L. lactis was able to secrete MPB70 using the L. lactis signal peptide Usp45, and to produce MPB83 as an immunogenic membrane protein following its expression with the signal peptide of the L. plantarum lipoprotein prsA. Inactivated cells of MPB83-expressing L. lactis cultures enhanced NF-κB activation in macrophages. Our results show that L. lactis is a reliable host for the secretion and functional expression of antigens that are naturally produced by M. bovis, the causative agent of bovine tuberculosis (bTB). This represents the first step on a long process to establishing whether recombinant LAB could serve as a food-grade platform for potential diagnostic tools and/or vaccine interventions for use against bTB, a chronic disease that primarily affects cattle but also humans and a wide range of domestic and wild animals.

Keywords: Antigen; Bovine tuberculosis; Functional expression; Lactic acid bacteria; Mycobacterium bovis; Secretion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / metabolism*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Cells, Cultured
  • DNA, Bacterial / biosynthesis
  • Gene Expression
  • Genetic Vectors / immunology
  • Humans
  • Lactobacillales / genetics
  • Lactobacillales / metabolism*
  • Lactobacillus plantarum / genetics
  • Lactobacillus plantarum / metabolism
  • Lactococcus lactis / genetics
  • Lactococcus lactis / metabolism
  • Macrophages / metabolism
  • Macrophages / microbiology
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mycobacterium bovis / immunology*
  • NF-kappa B / metabolism
  • Recombination, Genetic

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • DNA, Bacterial
  • MPB83 protein, Mycobacterium
  • Membrane Proteins
  • NF-kappa B
  • MPB70 protein, Mycobacterium bovis