Polyhydroxyalkanoates (PHA) production from fermented thermal-hydrolyzed sludge by mixed microbial cultures: The link between phosphorus and PHA yields

Abstract

Production of polyhydroxyalkanoates (PHA) from wastes has gained increasing attention for the related low costs and high environmental benefits. Phosphorus limitation is a potential strategy used to facilitate PHA production, yet excessive limitation was previously reported to cause negative effects. This study was the first to investigate the optimum phosphorus limitation for PHA accumulation from thermal-hydrolyzed sludge. The results showed that the maximum PHA content increased from 23 wt% to 51 wt% when phosphorus concentration was limited from 127.60 to 1.35 mg/L, indicating that a lower phosphorus concentration would promote maximum PHA accumulation. Batch tests performed with synthetic substrates (containing one specific VFA for each batch) confirmed that the effect of phosphorus content on PHA production was mainly devoted by the efficiency of the conversion of acetate to PHA. The PHA yields on acetate (Y_PHA/ac) were 0.68 and 0.05 Cmol/Cmol under phosphorus-limited (1 mg/L) and -excess (100 mg/L) conditions, respectively. A mathematical model was developed to describe the correlation between phosphorus concentration and Y_PHA/ac, which can fit the experimental data and predict the results properly. Finally, further (ammonium-) nitrogen restriction did not efficiently cause the additional improvement of PHA production under the conditions of phosphorus limitation.

Keywords: Mixed microbial cultures; Phosphorus limitation; Polyhydroxyalkanoates; Thermal hydrolysis sludge.