Non-Conventional Metal Ion Cofactor Requirement of Dinoflagellate Alkaline Phosphatase and Translational Regulation by Phosphorus Limitation

Xin Lin; Chentao Guo; Ling Li; Tangcheng Li; Senjie Lin

doi:10.3390/microorganisms7080232

Non-Conventional Metal Ion Cofactor Requirement of Dinoflagellate Alkaline Phosphatase and Translational Regulation by Phosphorus Limitation

Microorganisms. 2019 Aug 1;7(8):232. doi: 10.3390/microorganisms7080232.

Authors

Xin Lin¹, Chentao Guo², Ling Li², Tangcheng Li², Senjie Lin^{3

4}

Affiliations

¹ State Key Laboratory of Marine Environmental Science (MEL), Xiamen City Key Laboratory of Urban Sea Ecological Conservation and Restoration (USER), Xiamen University, Xiamen 361102, China. xinlin@xmu.edu.cn.
² State Key Laboratory of Marine Environmental Science (MEL), Xiamen City Key Laboratory of Urban Sea Ecological Conservation and Restoration (USER), Xiamen University, Xiamen 361102, China.
³ State Key Laboratory of Marine Environmental Science (MEL), Xiamen City Key Laboratory of Urban Sea Ecological Conservation and Restoration (USER), Xiamen University, Xiamen 361102, China. senjie.lin@uconn.edu.
⁴ Department of Marine Sciences, University of Connecticut, Groton, CT 06405, USA. senjie.lin@uconn.edu.

Abstract

Alkaline phosphatase (AP) enables marine phytoplankton to utilize dissolved organic phosphorus (DOP) when dissolved inorganic phosphate (DIP) is depleted in the ocean. Dinoflagellate AP (Dino-AP) represents a newly classified atypical type of AP, PhoA^aty. Despite While being a conventional AP, PhoA^EC is known to recruit Zn²⁺ and Mg²⁺ in the active center, and the cofactors required by PhoA^aty have been contended and remain unclear. In this study, we investigated the metal ion requirement of AP in five dinoflagellate species. After AP activity was eliminated by using EDTA to chelate metal ions, the enzymatic activity could be recovered by the supplementation of Ca²⁺, Mg²⁺ and Mn²⁺ in all cases but not by that of Zn²⁺. Furthermore, the same analysis conducted on the purified recombinant ACAAP (AP of Amphidinium carterae) verified that the enzyme could be activated by Ca²⁺, Mg²⁺, and Mn²⁺ but not Zn²⁺. We further developed an antiserum against ACAAP, and a western blot analysis using this antibody showed a remarkable up-regulation of ACAAP under a phosphate limitation, consistent with elevated AP activity. The unconventional metal cofactor requirement of Dino-AP may be an adaptation to trace metal limitations in the ocean, which warrants further research to understand the niche differentiation between dinoflagellates and other phytoplankton that use Zn-Mg AP in utilizing DOP.

Keywords: alkaline phosphatase; dinoflagellate; inducible expression; metal cofactor.

Abstract

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