To improve the utility of walnut meal protein, influences of ultrasonication on the kinetic and thermodynamic constraints of enzyme (trypsin) hydrolysis were assessed using a multi-frequency counter current S-type ultrasound machine. Results revealed that both the ultrasonication treatment and control hydrolysis obeyed the first-order kinetics within the study constraints. After 90 min hydrolysis time, the uppermost percentage conversion rate (57.5%) of substrate to product and the percentage increase (25.42%) in hydrolyzed protein concentration were obtained at 323 K and 10% substrate concentration, respectively. The Michaelis constant (KM ) in ultrasonic enzymolysis declined by 58.66% over the control. The reaction rate coefficient (k) for the proteolysis improved by 84.75%, 52.43%, 48.25%, and 37.79% at 219, 303, 313, and 323 K, correspondingly. Generally, the bond energy (Ea ), enthalpy change (ΔH), entropy change (ΔS), and Gibbs free energy (ΔG) were reduced by the ultrasound pretreatment with 36.61%, 28.05%, 18.22%, and 5.24%, respectively. PRACTICAL APPLICATIONS: Walnut meal protein has a well-balanced amino acid profile and its economic utilization could be increased practicably as a food ingredient via production of hydrolysates/peptides for the production of improved food ingredients instead of being cast off as animal feed. This study demonstrated a positive bearing of ultrasonication-assisted alkaline pretreatment on proteolytic reaction characteristics and its energy efficiency for walnut meal protein, which makes this technique applicable to the enhancement of plant proteins for inclusion in food products, especially tree nut and oil seed waste products from the oil industry.
Keywords: counter current S-type ultrasound; enzymolysis; kinetics; protein; walnut meal.
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