Viral etiology of acute respiratory infections in children in Southern Iran

Rev Soc Bras Med Trop. 2019 Jul 29:52:e20180249. doi: 10.1590/0037-8682-0249-2018.

Abstract

Introduction: Prevalence of influenza A virus (Flu-A), respiratory syncytial virus (RSV), and human metapneumovirus (hMPV) was assessed in children with acute respiratory infections (ARIs).

Methods: Nasopharyngeal aspirates and throat swabs were subjected to real-time polymerase chain reaction (PCR) to detect RSV and Flu-A and to conventional PCR to detect hMPV.

Results: Of the 156 children assessed, 93 (59.6%) carried at least one virus, with 35.9% positive for RSV, 14.1% for hMPV, and 9.6% for Flu-A. The prevalence of co-infections was 2.6%.

Conclusions: The high detection rate may reflect increased sensitivity of real-time PCR compared to traditional PCR and viral culture.

MeSH terms

  • Adolescent
  • Child
  • Child, Preschool
  • Cross-Sectional Studies
  • Female
  • Humans
  • Influenza A virus / genetics
  • Influenza, Human / epidemiology*
  • Iran / epidemiology
  • Male
  • Metapneumovirus / genetics
  • Nasopharynx / virology
  • Paramyxoviridae Infections / epidemiology*
  • Real-Time Polymerase Chain Reaction
  • Respiratory Syncytial Virus Infections / epidemiology*
  • Respiratory Syncytial Virus, Human / genetics
  • Respiratory Tract Infections / epidemiology
  • Respiratory Tract Infections / virology*