Enhanced Binding Efficiency of Microcantilever Biosensor for the Detection of Yersinia

Xiaochen Liu; Lihao Wang; Junyuan Zhao; Yinfang Zhu; Jinling Yang; Fuhua Yang

doi:10.3390/s19153326

Enhanced Binding Efficiency of Microcantilever Biosensor for the Detection of Yersinia

Sensors (Basel). 2019 Jul 29;19(15):3326. doi: 10.3390/s19153326.

Authors

Xiaochen Liu^{1

2

3}, Lihao Wang^{1

2

3}, Junyuan Zhao^{1

2

3}, Yinfang Zhu^{4

5

6}, Jinling Yang^{7

8

9}, Fuhua Yang^{1

2}

Affiliations

¹ Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.
² Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Science, Beijing 100049, China.
³ State Key Laboratory of Transducer Technology, Shanghai 200050, China.
⁴ Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China. yfzhu@semi.ac.cn.
⁵ Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Science, Beijing 100049, China. yfzhu@semi.ac.cn.
⁶ State Key Laboratory of Transducer Technology, Shanghai 200050, China. yfzhu@semi.ac.cn.
⁷ Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China. jlyang@semi.ac.cn.
⁸ Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Science, Beijing 100049, China. jlyang@semi.ac.cn.
⁹ State Key Laboratory of Transducer Technology, Shanghai 200050, China. jlyang@semi.ac.cn.

Abstract

A novel microcantilever sensor was batch fabricated for Yersinia detection. The microcantilever surface modification method was optimized by introducing a secondary antibody to increase the number of binding sites. A novel microfluidic platform was designed and fabricated successfully. A 30 μL solution could fully react with the microcantilever surface. Those routines enhanced the binding efficiency between the target and receptor on the microcantilever. With this novel designed microfluidic platform, the specific adsorption of 10⁷ Yersinia on the beam surface with modified F1 antibody was significantly enhanced.

Keywords: Yersinia; binding efficiency; microcantilever; microfluidic system.

MeSH terms

Antibodies / chemistry*
Antibodies / immunology
Binding Sites
Biosensing Techniques*
Humans
Microfluidics / methods
Surface Properties
Yersinia / chemistry
Yersinia / immunology
Yersinia / isolation & purification*
Yersinia Infections / diagnosis*
Yersinia Infections / immunology
Yersinia Infections / microbiology

Substances

Antibodies