MiR-146a mimic attenuates murine allergic rhinitis by downregulating TLR4/TRAF6/NF-κB pathway

Immunotherapy. 2019 Sep;11(13):1095-1105. doi: 10.2217/imt-2019-0047. Epub 2019 Jul 30.

Abstract

Aim: This study aims to investigate whether microRNA (miR)-146a exerts therapeutic potential to allergic rhinitis (AR). Methods: Female BALB/c mice were randomly divided into the AR and miR-146a groups. miR-146a was administered into the nostril before ovalbumin (OVA) challenge daily on days 21-28. Results: The expression level of miR-146a in AR nasal mucosa was significantly decreased. miR-146a mimic markedly attenuated sneezing and nasal rubbing events, decreased the levels of ovalbumin-specific IgE as well as mastocyte- and basophil-related inflammatory cytokines, reduced inflammatory cells, affected the T-helper 2-released cytokines. Furthermore, miR-146a mimic inhibited the expressions of proteins relevant to Toll-like receptor 4 (TLR4)/TRAF6/NF-κB signaling pathway. Conclusion: The anti-inflammatory effects of miR-146a on AR might exert through the inhibition of the toll-like receptor 4 (TLR4)/TRAF6/NF-κB signaling pathway to some extent.

Keywords: NF-κB; TLR4; TRAF6; allergic rhinitis; miR-146a.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / immunology
  • Animals
  • Anti-Inflammatory Agents / therapeutic use*
  • Biomimetics / methods
  • Disease Models, Animal
  • Female
  • Humans
  • Immunoglobulin E / blood
  • Mice
  • Mice, Inbred BALB C
  • MicroRNAs / genetics*
  • MicroRNAs / therapeutic use*
  • NF-kappa B / metabolism
  • Nasal Mucosa / metabolism*
  • Nasal Mucosa / pathology
  • Ovalbumin / immunology
  • Rhinitis, Allergic / genetics
  • Rhinitis, Allergic / therapy*
  • Signal Transduction
  • TNF Receptor-Associated Factor 6 / metabolism
  • Toll-Like Receptor 4 / metabolism

Substances

  • Allergens
  • Anti-Inflammatory Agents
  • MicroRNAs
  • Mirn146 microRNA, mouse
  • NF-kappa B
  • TNF Receptor-Associated Factor 6
  • Tlr4 protein, mouse
  • Toll-Like Receptor 4
  • Immunoglobulin E
  • Ovalbumin