Headspace Volatile Evaluation of Carrot Samples-Comparison of GC/MS and AuNPs-hpDNA-Based E-Nose

Sara Gaggiotti; Marcello Mascini; Paola Pittia; Flavio Della Pelle; Dario Compagnone

doi:10.3390/foods8080293

Headspace Volatile Evaluation of Carrot Samples-Comparison of GC/MS and AuNPs-hpDNA-Based E-Nose

Foods. 2019 Jul 27;8(8):293. doi: 10.3390/foods8080293.

Authors

Sara Gaggiotti¹, Marcello Mascini¹, Paola Pittia¹, Flavio Della Pelle², Dario Compagnone³

Affiliations

¹ Faculty of Bioscience and Technology for Food, Agriculture, and Environment, University of Teramo, via Renato Balzarini 1, 64100 Teramo, Italy.
² Faculty of Bioscience and Technology for Food, Agriculture, and Environment, University of Teramo, via Renato Balzarini 1, 64100 Teramo, Italy. fdellapelle@unite.it.
³ Faculty of Bioscience and Technology for Food, Agriculture, and Environment, University of Teramo, via Renato Balzarini 1, 64100 Teramo, Italy. dcompagnone@unite.it.

Abstract

The performances of a quartz crystal microbalances (QCMs) based on an electronic nose (E-nose), modified with hairpin-DNA (hpDNA) for carrot aroma profiling has been evaluated. Solid phase micro-extraction (SPME) headspace sampling, combined with gas chromatography (GC), was used as a reference method. The changes in carrot aroma profiles stored at different temperatures (-18 °C, 4 °C, 25 °C, and 40 °C) were monitored during time up to 26 days. The principal component analysis of the data evidenced the different aroma patterns related to the presence of different key compounds. The output data achieved with the hpDNA-based E-nose were able to detect aroma patterns similar to gas chromatography with mass spectrometry (GC-MS). This work demonstrates that hpDNA has different sizes of loops that can be used for the development of sensor arrays able to detect aroma patterns in food and their changes with advantages in terms of easiness of usage, rapidity, and cost of analysis versus classical methods.

Keywords: carrots; electronic nose; gold nanoparticles-hairpin-DNA (AuNPs-hpDNA); quartz crystal microbalances (QCMs); sensor array; volatile compounds analysis.