Artesunate-luminol chemiluminescence system for the detection of hemin

Tadesse Haile Fereja; Shimeles Addisu Kitte; Wenyue Gao; Fan Yuan; Dmytro Snizhko; Liming Qi; Anaclet Nsabimana; Zhongyuan Liu; Guobao Xu

doi:10.1016/j.talanta.2019.06.007

Artesunate-luminol chemiluminescence system for the detection of hemin

Talanta. 2019 Nov 1:204:379-385. doi: 10.1016/j.talanta.2019.06.007. Epub 2019 Jun 4.

Authors

Tadesse Haile Fereja¹, Shimeles Addisu Kitte², Wenyue Gao², Fan Yuan³, Dmytro Snizhko⁴, Liming Qi², Anaclet Nsabimana², Zhongyuan Liu⁵, Guobao Xu⁶

Affiliations

¹ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China; Ambo University, College of Medicine and Health Sciences, Department of Pharmacy, P.O.Box 19, Ambo, Ethiopia.
² State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
³ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, PR China; University of Science and Technology of China. Anhui, 230026, PR China.
⁴ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, PR China; Laboratory of Analytical Optochemotronics, Kharkiv National University of Radio Electronics, 14 Nauka Ave., Kharkiv, 61166, Ukraine.
⁵ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, PR China. Electronic address: zhongyuanliu@ciac.ac.cn.
⁶ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, PR China; University of Science and Technology of China. Anhui, 230026, PR China. Electronic address: guobaoxu@ciac.ac.cn.

PMID: 31357309
DOI: 10.1016/j.talanta.2019.06.007

Abstract

Fabricating simple, accurate and user-friendly diagnostic device for "point of care testing" (POCT) applications is one of the most challenging objectives in the analytical field. Hemin detection is important for drugs monitoring, diagnosis, and forensic latent bloodstain imaging. Herein is developed, luminol chemiluminescence biosensor for hemin detection using artesunate as coreactant. A possible mechanism to account for the chemiluminescence reaction is discussed. Hemin was detected using both photomultiplier tube (PMT) and smartphone as detector. The detection limit for hemin using smartphone as detector is 20 nM, enabling the visual detection of hemin in blood sample with a dilution factor of blood up to 120,000. While PMT detector is used, the system is able to detect hemin down to 0.22 nM. In addition to high sensitivity, this sensing system exhibit high selectivity. It can successfully distinguish bloodstain from other stains while applying the system for point of care testing using smart phone as detector. Moreover, the system can detect artesunate with a linear range from 0.1 nM to 1.0 μM with a limit of detection of 0.078 nM.

Keywords: Artesunate; Chemiluminescence; Hemin; Point of care testing; Smartphone.

MeSH terms

Artesunate / analysis
Artesunate / chemistry*
Blood Chemical Analysis / instrumentation
Blood Chemical Analysis / methods
Blood Stains
Calibration
Hemin / analysis*
Humans
Limit of Detection
Luminescent Agents / chemistry*
Luminescent Measurements / instrumentation
Luminescent Measurements / methods
Luminol / chemistry*
Smartphone

Substances

Luminescent Agents
Luminol
Artesunate
Hemin