A secreted WY-domain-containing protein present in European isolates of the oomycete Plasmopara viticola induces cell death in grapevine and tobacco species

PLoS One. 2019 Jul 29;14(7):e0220184. doi: 10.1371/journal.pone.0220184. eCollection 2019.

Abstract

Plasmopara viticola is a biotrophic oomycete pathogen causing grapevine downy mildew. We characterized the repertoire of P. viticola effector proteins which may be translocated into plants to support the disease. We found several secreted proteins that contain canonical dEER motifs and conserved WY-domains but lack the characteristic RXLR motif reported previously from oomycete effectors. We cloned four candidates and showed that one of them, Pv33, induces plant cell death in grapevine and Nicotiana species. This activity is dependent on the nuclear localization of the protein. Sequence similar effectors were present in seven European, but in none of the tested American isolates. Together our work contributes a new type of conserved P. viticola effector candidates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Death
  • Cell Nucleus / metabolism
  • Cloning, Molecular
  • Europe
  • Evolution, Molecular
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism*
  • Host-Pathogen Interactions
  • Nicotiana / microbiology*
  • Peronospora / classification
  • Peronospora / isolation & purification*
  • Peronospora / metabolism
  • Phylogeny
  • Plant Diseases / microbiology
  • Protein Domains
  • Sequence Analysis, Protein
  • Species Specificity
  • United States
  • Vitis / microbiology*

Substances

  • Fungal Proteins

Grants and funding

Research was funded by Agence Nationale de la Recherche (ANR) grant EFFECTOORES (ANR-13-ADAP-0003) PM.(https://anr.fr/). MC was funded by a joint fellowship from INRA BAP Department (http://www.bap.inra.fr/) and Region Alsace (https://www.grandest.fr/). EE and SS are funded by the Gatsby Charitable Foundation (https://www.gatsby.org.uk/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.