The aim of this study is to investigate the protective effect of green tea (GT) against the toxicity of nicotine. BALB/c mice were divided into four groups. Group I received food and water intake ad libidium, Group II received GT solution at a dose of 1 ml/kg body weight orally twice a day via gastric gavage, Group III was injected intraperitoneally with nicotine (2.5 mg/kg) once per day for 4 weeks, and Group IV received both nicotine and GT; GT was introduced using gastric gavage 1 hr before and 1 hr after the nicotine injection. The administration of nicotine altered the cellular antioxidant defense system by inducing inflammation and damage in the tissues of liver, lungs, and kidneys. In addition, nicotine treatment significantly enhanced the expression antioxidant- and inflammation-related genes. There were significant improvements when the nicotine-exposed mice treated with GT. PRACTICAL APPLICATIONS: In this study, it is revealed that the administration of nicotine altered the cellular antioxidant defense system by inducing inflammation manifested by the infiltration of inflammatory cells and damage seen in liver, lungs, and kidneys. GT contributed to the reduction of toxicity of nicotine, probably mediated by free radicals, through downregulation of nicotine-induced upregulated antioxidant- and inflammation-related genes. Never the less, further in depth investigation on characterization of the active constituents of GT responsible for their effect seen here and the mechanism that contributes to the effects seen in this reports is highly demanded. Furthermore, GT extract could be considered as a dietary supplement for the reduction of nicotine toxicity among cigarette smoker.
Keywords: antioxidant-related genes; green tea; inflammation-related genes; nicotine; oxidative stress.
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