Recent developments in spontaneous Raman imaging of living biological cells

Bianca Durrant; Matthew Trappett; Dustin Shipp; Ioan Notingher

doi:10.1016/j.cbpa.2019.06.004

Recent developments in spontaneous Raman imaging of living biological cells

Curr Opin Chem Biol. 2019 Aug:51:138-145. doi: 10.1016/j.cbpa.2019.06.004. Epub 2019 Jul 22.

Authors

Bianca Durrant¹, Matthew Trappett¹, Dustin Shipp¹, Ioan Notingher²

Affiliations

¹ Department of Physics, Utah Valley University, 800 West University Parkway, Orem, UT 84058, United States.
² School of Physics and Astronomy, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom. Electronic address: ioan.notingher@nottingham.ac.uk.

PMID: 31344641
DOI: 10.1016/j.cbpa.2019.06.004

Abstract

Raman spectroscopy is a non-invasive optical technique for measuring chemical information from samples including biological cells. With its high sensitivity and compatibility with in vitro samples, Raman spectroscopy is increasing in popularity as a tool for acquiring chemical images of cellular samples in fields including parasitology, pharmacology, and oncology. This review describes emerging technologies that are improving the speed, resolution, and impact of Raman imaging techniques. Topics include novel instrumentation for data acquisition and improvements on techniques for displaying hyperspectral data as images.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Multivariate Analysis
Spectrum Analysis, Raman / methods*