This document addresses the susceptibility testing of Mycobacterium tuberculosis complex (MTBC), clinically significant slowly and rapidly growing mycobacterial species, Nocardia spp., and other aerobic actinomycetes. Included in this standard are recommendations for the selection of agents for primary and secondary testing, organism group–specific methodologies, reporting recommendations, and quality control criteria for the above-listed organisms. Recommendations regarding the selection of agents for testing mycobacteria are based primarily on guidelines from US agencies. For testing MTBC, M24 recognizes agar proportion as the primary methodology on which all other methodologies are essentially based. This document also includes recommendations for use of commercial broth susceptibility methods with shorter incubation times, which are now in widespread use in the susceptibility testing of MTBC.
Clinical and Laboratory Standards Institute (CLSI). Susceptibility Testing of Mycobacteria, Nocardiae, and Other Aerobic Actinomycetes; Approved Standard—Second Edition. CLSI document M24-A2 (ISBN 1-56238-746-4). Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087 USA, 2011.
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