A Gram-stain-negative, rod-shaped and facultative anaerobic strain named YE-B6T was isolated from the intestine of abalone, Haliotis discus hannai. The reactions of oxidase and catalase were both positive. Strain YE-B6T could grow at 10-37 °C (optimum, 28-33 °C), at pH 7-9 (optimum, pH 7) and in salinity of 0-8 % NaCl (w/v; optimum, 2 %). The 16S rRNA gene sequence of strain YE-B6T had maximum sequence similarities to Cohaesibacter celericrescens H1304T (98.0 %), Cohaesibacter marisflavi DQHS21T (97.4 %), Cohaesibacter haloalkalitolerans JC131T (97.4 %) and Cohaesibacter gelatinilyticus CL-GR15T (97.3 %). Results of phylogenetic analysis showed that it was affiliated to the genus Cohaesibacter within the order Rhizobiales in the Alphaproteobacteria. The respiratory quinone of strain YE-B6T was Q-10. The cellular fatty acids mainly comprised C18 : 1 ω7c and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, two unidentified aminolipids, two unidentified polar lipids and one unidentified phospholipid. The G+C content of strain YE-B6T was 55.6 mol%. Based on its genotypic, physiological and biochemical characteristics, strain YE-B6T represented a novel species within the genus Cohaesibacter, for which the name Cohaesibacterintestini sp. nov. is proposed. The type strain of the new species is YE-B6T (=MCCC 1A13131T=KCTC 62716T).
Keywords: Cohaesibacter intestini; Haliotis discus hannai; polyphasic taxonomy.