AS1411-conjugated gold nanoparticles affect cell proliferation through a mechanism that seems independent of nucleolin

Nanomedicine. 2019 Oct:21:102060. doi: 10.1016/j.nano.2019.102060. Epub 2019 Jul 20.

Abstract

G-rich oligonucleotide, AS1411, has been shown to interact with nucleolin and to inhibit cancer cell proliferation and tumor growth. This antiproliferative action is increased when AS1411 is conjugated to different types of nanoparticles. However, the molecular mechanisms are not known. In this work, we show in several cell lines that optimized AS1411-conjugated gold nanoparticles (GNS-AS1411) inhibit nucleolin expression at the RNA and protein levels. We observed an alteration of the nucleolar structure with a decrease of ribosomal RNA accumulation comparable to what is observed upon nucleolin knock down. However, the expression of genes involved in cell cycle and the cell cycle blockage by GNS-AS1411 are not regulated in the same way as that in cells where nucleolin has been knocked down. These data suggest that the anti-proliferative activity of GNS-AS1411 is not the only consequence of nucleolin targeting and down-regulation.

Keywords: AS1411 aptamer; Cancer; Gold nanoparticles; Nucleolin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide
  • Cell Cycle Checkpoints / drug effects*
  • Cell Line, Tumor
  • Down-Regulation / drug effects*
  • Gold* / chemistry
  • Gold* / pharmacology
  • Humans
  • Metal Nanoparticles / chemistry*
  • Nucleolin
  • Oligodeoxyribonucleotides* / chemistry
  • Oligodeoxyribonucleotides* / pharmacology
  • Phosphoproteins / biosynthesis*
  • RNA, Ribosomal / biosynthesis*
  • RNA-Binding Proteins / biosynthesis*

Substances

  • AGRO 100
  • Aptamers, Nucleotide
  • Oligodeoxyribonucleotides
  • Phosphoproteins
  • RNA, Ribosomal
  • RNA-Binding Proteins
  • Gold