A novel approach to improving hybrid capture sequencing targeting efficiency

WenXiang Lu; Miao Zhu; Yi Chen; Yunfei Bai

doi:10.1016/j.mcp.2019.101424

A novel approach to improving hybrid capture sequencing targeting efficiency

Mol Cell Probes. 2019 Aug:46:101424. doi: 10.1016/j.mcp.2019.101424. Epub 2019 Jul 20.

Authors

WenXiang Lu¹, Miao Zhu², Yi Chen³, Yunfei Bai⁴

Affiliations

¹ Department of R&D, Decode Genomics Incorporation, Nanjing, 210000, China; Department of R&D, AGCU ScienTech Incorporation, Wuxi, 214174, China.
² Department of R&D, Decode Genomics Incorporation, Nanjing, 210000, China.
³ Department of Obstetrics and Gynaecology, Kunshan Hospital of Traditional Chinese Medicine, Kunshan, 215300, China. Electronic address: chenyi201988@163.com.
⁴ State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering, Southeast University, Nanjing 210096, China. Electronic address: whitecf@seu.edu.cn.

PMID: 31336168
DOI: 10.1016/j.mcp.2019.101424

Abstract

At present, approaches to hybrid capture sequencing have many limitations, from their significant complexity and labor requirements, to their low enrichment efficiency, resulting in their limited utilization. In an effort to overcome these drawbacks, we have developed a novel method that relied upon direct genomic DNA hybridization and single-stranded DNA library preparation. Using this novel protocol, we were able to achieve a targeting efficiency as high as 75%, and we found this approach to overall be an efficient and simple approach to DNA library construction.

Keywords: Hybrid capture; Library construction; Single-stranded DNA; Targeting efficiency.

MeSH terms

DNA / genetics
Gene Library
Genome, Human / genetics*
Genomics*
High-Throughput Nucleotide Sequencing*
Humans
Nucleic Acid Hybridization*
Sequence Analysis, DNA

Substances

DNA