Two new phytoecdysteroids from Sphenocentrum jollyanum Pierre root

Temitayo O Ajayi; Radhakrishnan Srivedavyasasri; Emmanuel E Nyong; Micheal A Odeniyi; Jones O Moody; Samir A Ross

doi:10.1016/j.steroids.2019.108456

Two new phytoecdysteroids from Sphenocentrum jollyanum Pierre root

Steroids. 2019 Oct:150:108456. doi: 10.1016/j.steroids.2019.108456. Epub 2019 Jul 19.

Authors

Temitayo O Ajayi¹, Radhakrishnan Srivedavyasasri², Emmanuel E Nyong³, Micheal A Odeniyi⁴, Jones O Moody⁵, Samir A Ross⁶

Affiliations

¹ National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA; Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Nigeria.
² National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA. Electronic address: svsrkb@olemiss.edu.
³ Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Nigeria; Department of Pharmacognosy and Natural Medicine, University of Uyo, Akwa Ibom State, Nigeria.
⁴ Department of Pharmaceutics, Faculty of Pharmacy, University of Ibadan, Nigeria.
⁵ Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Nigeria.
⁶ National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA; BioMolecular Sciences, Division of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS 38677, USA. Electronic address: sross@olemiss.edu.

PMID: 31326452
DOI: 10.1016/j.steroids.2019.108456

Abstract

The crude methanol extract of Sphenocentrum jollyanum root exhibited 98% and 80% antimicrobial activity against Aspergillus fumigatus Pinh and Vancomycin resistant enterococcus (VRE) at a concentration of 200 µg/mL, with IC₅₀ 11.45 and 12.95 µg/mL, respectively. The ethyl acetate fraction of methanol extract showed in-vitro antimicrobial activity against A. fumigatus Pinh at 83% with IC₅₀ of <8 µg/mL. The phytochemical investigation of ethyl acetate fraction yielded six compounds, which were identified by their NMR, IR and MS spectral analyses as two new phytoecdysteroidal glycosides Sphenocentroside A (1), and Sphenocentroside B (2), and four known phytoecdysteroids: polypodoaurein (3), polypodine B (4), ecdysterone (5), and 20, 26-dihydroxyecdysone (6).

Keywords: Antimicrobial activity; Aspergillus fumigatus; Phytoecdysteroids; Sphenocentrum jollyanum; Vancomycin resistant enterococcus.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / isolation & purification
Anti-Bacterial Agents / pharmacology*
Antifungal Agents / chemistry
Antifungal Agents / isolation & purification
Antifungal Agents / pharmacology*
Aspergillus fumigatus / drug effects
Dose-Response Relationship, Drug
Ecdysterone / chemistry
Ecdysterone / isolation & purification
Ecdysterone / pharmacology*
Menispermaceae / chemistry*
Microbial Sensitivity Tests
Molecular Conformation
Plant Extracts / chemistry*
Plant Extracts / isolation & purification
Plant Extracts / pharmacology*
Plant Roots / chemistry*
Stereoisomerism
Structure-Activity Relationship
Vancomycin-Resistant Enterococci / drug effects

Substances

Anti-Bacterial Agents
Antifungal Agents
Plant Extracts
Ecdysterone