Endogenous protection against the 6-OHDA model of Parkinson's disease in the Amazonian rodent Proechimys

Marcia Jonathas Guimarães Marques; Caroline Cristiano Real; Daniella Balduino Victorino; Luiz Roberto Britto; Esper Abrão Cavalheiro; Fulvio Alexandre Scorza; Henrique Ballalai Ferraz; Carla Alessandra Scorza

doi:10.1016/j.neulet.2019.134381

Endogenous protection against the 6-OHDA model of Parkinson's disease in the Amazonian rodent Proechimys

Neurosci Lett. 2019 Sep 14:709:134381. doi: 10.1016/j.neulet.2019.134381. Epub 2019 Jul 17.

Authors

Marcia Jonathas Guimarães Marques¹, Caroline Cristiano Real², Daniella Balduino Victorino¹, Luiz Roberto Britto³, Esper Abrão Cavalheiro¹, Fulvio Alexandre Scorza¹, Henrique Ballalai Ferraz⁴, Carla Alessandra Scorza⁵

Affiliations

¹ Discipline of Neuroscience, Department of Neurology and Neurosurgery, Federal University of São Paulo Paulista Medical School, São Paulo, SP, Brazil.
² Laboratory of Cellular Neurobiology, Department of Biophysics and Physiology, University of São Paulo, São Paulo, SP, Brazil; Laboratory of Nuclear Medicine (LIM 43), Department of Radiology and Oncology, Faculty of Medicine of the University of São Paulo, São Paulo, SP, Brazil.
³ Laboratory of Cellular Neurobiology, Department of Biophysics and Physiology, University of São Paulo, São Paulo, SP, Brazil.
⁴ Movement Disorders Unit, Department of Neurology and Neurosurgery, Federal University of São Paulo Paulista Medical School, São Paulo, SP, Brazil.
⁵ Discipline of Neuroscience, Department of Neurology and Neurosurgery, Federal University of São Paulo Paulista Medical School, São Paulo, SP, Brazil. Electronic address: carla.scorza@unifesp.br.

PMID: 31325585
DOI: 10.1016/j.neulet.2019.134381

Abstract

Background: Proechimys, an epilepsy-resistant rodent from Amazon Rainforest, is a promising alternative animal model for studying neurodegenerative disorders.

Objectives: To evaluate behavioral and immunohistological changes in Proechimys after 6-OHDA-induced model of PD.

Methods: Following unilateral injections of 6-OHDA into striatum, animals were assessed for exploratory behavior using the cylinder test. Brain sections were submitted to immunohistochemistry for tyrosine hydroxylase (TH), glial fibrillary acidic protein (GFAP), and ionized calcium-binding adaptor molecule 1 (Iba-1).

Results: We observed normal exploratory behavior during cylinder test in all animals. We could not detect changes in the expression of TH in both striatum and SNc, suggesting that Proechimys is resistant to dopaminergic neuronal degeneration. Glial activation was observed by an increase in Iba-1 expression in both striatum and SNc, and by an increase in GFAP expression in striatum.

Conclusions: Proechimys represents a promising animal model for studying the mechanisms underlying the susceptibility of dopaminergic neurons to degeneration induced by 6-OHDA.

Keywords: 6-hydroxydopamine; Neuroprotection; Parkinson´s disease; Proechimys.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Corpus Striatum / drug effects
Corpus Striatum / pathology
Disease Models, Animal*
Exploratory Behavior / drug effects
Exploratory Behavior / physiology*
Male
Neuroprotection / physiology*
Oxidopamine / toxicity*
Parkinsonian Disorders / chemically induced*
Parkinsonian Disorders / pathology*
Parkinsonian Disorders / prevention & control
Rats
Rodentia
Stereotaxic Techniques

Substances

Oxidopamine