Epiphytic bacterial community shift drives the nutrient cycle during Potamogeton malaianus decomposition

Abstract

Epiphytic bacteria on submerged macrophytes play important roles in the nutrient cycle in freshwater ecosystems. However, little is known about the composition and role of epiphytic bacteria during the decomposition of submerged macrophytes. In this study, the alterations in epiphytic bacterial composition, abundances of nitrogen cycle-related genes and nutrient release were investigated in a 56-day decomposition process of Potamogeton malaianus. The total reduced biomass was positively related to the contents of carbon, nitrogen and phosphorus released from plant residues. Nutrient released from plant litter showed a positively effect on the concentrations of carbon, nitrogen and phosphorus in the overlying water (p < 0.01). The carbon, phosphorus and nitrogen decreased with decomposition process in both plant debris and overlying water. Humic acid-like substances were the main component of dissolved organic matter in the conditioning stage, whereas fulvic acid-like substances dominated in the fragmentation stage. Results from network analysis and canonical correspondence analysis showed dominant bacterial clades changed with decomposition process. Bacteroidetes was the most abundant phylum in the leaching stage and Spirochaetes, Chlorobi, and Bacteroidetes dominated in the conditioning stage, while Chlorobi dominated in the fragmentation stage. The highest abundance of cnorB and nosZ were detected in the leaching and fragmentation stage, respectively. Bacterial denitrification contributed to nitrogen removal and might be promoted by high ORP and DOC concentration. Our results indicate that epiphytic bacterial community shift drived the metabolism of nutrients C, N, and S during the decomposition of P. malaianus.

Keywords: Decomposition; Epiphytic bacteria; Nutrient cycle; Submerged macrophyte.