A tracking-based nanoimaging method for fast detection of surfaces' inhomogeneities using gold nanoparticles

Microsc Res Tech. 2019 Nov;82(11):1835-1842. doi: 10.1002/jemt.23350. Epub 2019 Jul 18.

Abstract

The localization of surfaces inhomogeneities is central to many areas of technology, chemistry and biology, ranging from surface defects in industry to the identification and screening of early bio-defects inside cells. The development of methods that enable direct, sensitive, and rapid detection of those inhomogeneities is both relevant and timely. To address this challenge, we developed a far-field nanoimaging method to detect the presence of surface's nanodefects that modify the signal emitted by gold nanoparticles (AuNPs) under laser irradiation. Our technique is based on the formation of hot spots due to the confinement of light in the proximity of the AuNP, whose positions depend on the polarization direction of the incident beam. An inhomogeneity is detected as an increase in the intensity collected from the hot spots when a laser beam is orbiting the nanoparticle and the incident polarization direction of the laser beam is changed periodically.

Keywords: inhomogeneities detection; microscopy; nanoparticles; tracking.