Keystone taxa regulate microbial assemblage patterns and functional traits of different microbial aggregates in simultaneous anammox and denitrification (SAD) systems

Abstract

Understanding the impacts of the ecological patterns and niche-based selection on microbial community assembly and nitrogen-cycling network is crucial for achieving energy-neutral wastewater treatment. However, little is known about the niche differentiation and microbial nitrogen-cycling traits of keystone taxa in flocs and granules in anammox-based systems. Herein, the aspects of community assemblage patterns, metabolic functions and nitrogen transformation pathways were explored. The findings discovered that the treatment performance and bacterial community assembly were regulated by core taxa and flocs and granules communities harbored core taxa based on their functional traits. Both niche differentiation and environmental filtering have profound influences on functional bacteria. Furthermore, a combined analysis showed that nitrogen removal in flocs and granules was regulated by different nitrogen transformation pathways. These results suggest that core taxa are the key drivers for the microbial nitrogen-cycling network and improve the understanding of cross-feeding and metabolic pathways between anammox and nitrogen-cycling-related microorganisms.

Keywords: Anammox; Community assemblage; Core taxa; Functional attributes; Microbial aggregates.