Reductive dechlorination of trichloroethene under different sulfate-reducing and electron donor conditions

Abstract

The effect of sulfate presence on reductive dechlorination of chlorinated ethenes has been a matter of conflict among the limited reports found in literature. This paper aims to clarify the misconceptions regarding the performance of trichloroethene biotransformation under sulfate reducing conditions by evaluating the effect of different sulfate concentrations on reductive dechlorination and to assess the influence of electron donor dose on dechlorination rate. To this end, batch experiments containing different sulfate and butyrate concentrations were conducted using trichloroethene-dechlorinating and sulfate-reducing parent cultures. Results demonstrated that if sufficient time and electron donor is provided, complete dechlorination can be achieved, even at up to 400 mg/L initial sulfate concentration. However, the rate of dichloroethene and vinyl chloride degradation is reduced as sulfide concentration increases. Moreover, the excess electron donor dose induced a slightly slower dechlorination rate. The findings of this paper present an explanatory framework for the dechlorination of TCE under sulfate reducing conditions and can contribute to the state-of-art bioremediation of contaminated sites.

Keywords: Dechlorination; Sulfate reduction; Sulfide inhibition; Trichloroethene (TCE).